Figure 2.

Treatment of COLO 205 cells with denbinobin (10-20 μmol/L) for 24 h induced apoptosis. A: FACS analysis of DNA content after 24-h incubation in culture medium supplemented with 10% FCS and 0.1% DMSO or 0-20 μmol/L denbinobin in 0.1% DMSO. Percentages of cells in sub-G₁, G₀/G₁, S, and G₂/M phases of the cell cycle determined using established CellFIT DNA analysis software were shown. Increased sub-G₁ percentage of COLO 205 cells was shown after treatment with 10-20 μmol/L denbinobin. Two independent experiments were performed in triplicate. ^aP<0.05 vs DMSO control. B: Electrophoresis of genomic DNA from COLO 205 treated with denbinobin. A typical DNA ladder pattern associated with apoptosis was seen at 10-20 μmol/L denbinobin.