Table 2.
PCR primer pairs for genotyping in cytokines gene.
| Gene | Position | Primers | Product size (bp) |
| IL-1B | +3953 | 5'-GTTGTCATCAGACTTTGACC-3’ | 249 |
| 5'-TTCAGTTCATATGCACCAGA-3’ | |||
| IL-1RN | exon2 | 5'-GCCCCTCAGCAACACTCCTAT-3’ | |
| 5'-CATCTTCCTGGTCTGCAGG-3’ | 1 | ||
| 5'-CATCTTCCTGGTCTGCAGC-3’ | |||
| IL-6 | -174G/C | 5'-CCCTAGTTGTGTCTTGCC-3’ | 190 |
| 5'-CCCTAGTTGTGTCTTGCG-3’ | 190 | ||
| 5'-GAGCTTCTCTTTCGTTCC-3’ | |||
| IL-10 | -1082G/A | 5'-TCTTACCTATCCCTACTTCC-3 | 139 |
| 5'-CTCGCTGCAACCCAACTGGC-3’ | |||
| -819T/C | 5'-AACTGAGGCACAGAGATG-3 | 402 | |
| 5'-AACTGAGGCACAGAGATA-3 | 402 | ||
| 5'-AGCAACACTCCTCGCCGCAAC-3’ | |||
| 592A/C | 5'-GGTGAGCACTACCTGACTAGC-3 | 412 | |
| 5'-CCTAGGTCACAGTGACGTGG-3’ | |||
| HLA-DRB1 | 5c | 5'- TGC CAA GTG GAG CAC CCA A-3 | 7962 |
| 3c | 5'-GCA TCT TGC TCT GTG CAG AT-3’ |
1Variable numbers of an 86 bp tandem repeat sequence.
2
The inter-reference of allele-specific PCR.