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. 2015 Jan 26;5:8008. doi: 10.1038/srep08008

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Copyright © 2015, Macmillan Publishers Limited. All rights reserved

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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(A) Human neutrophils were incubated with acapsular CAP67 and/or DPI or chloro-amidine (ClA). (B, C) Human neutrophils were preincubated with DPI (10 μg/mL), followed by DHR or H2DCFDA, washed and incubated with CAP67 yeast or PMA. The data are presented as arbitrary fluorescence units (AU) of three experiments. (D) Lactate dehydrogenase assay of neutrophils alone (control) or in the presence of DPI or ClA. The results represent the means ± SD of 4 donors performed in duplicate. +p<0.05 unstimulated control (CTR) versus CAP67 or PMA; *p<0.05 comparing CAP67 to CAP67 + DPI; #p<0.05 CAP67 versus CAP67 + ClA and ∧p<0.05 PMA compared to PMA + DPI.