Table 1. Kinetic parameters of recombinant MakMvan and MakMtb.
| Maltose | ATP | GTP | UTP | ||
|---|---|---|---|---|---|
| Enzyme | (with 5 mM ATP) | (with 20 mM Maltose) | |||
| KM (mM) | MakMtb* | 2.5 ± 0.4 | 0.7 ± 0.1 | 1.0 ± 0.2 | 1.3 ± 0.1 |
| MakMvan | 7.3 ± 0.9 | 2.6 ± 0.5 | 5.8 ± 1.4 | ND | |
| Vmax (μmol/min.mg) | MakMtb* | 21 ± 1 | 21 ± 1 | 19 ± 1 | 7.1 ± 0.3 |
| MakMvan | 301 ± 14 | 209 ± 19 | 62 ± 8 | ND | |
| Kcat (min −1) | MakMtb* | 52 ± 3 | 54 ± 2 | ND | ND |
| MakMvan | 3006 ± 139 | 2091 ± 188 | 616 ± 78 | ND | |
| Kcat/KM (mM −1.min−1) | MakMtb* | 21 ± 4 | 72 ± 12 | ND | ND |
| MakMvan | 414 ± 54 | 810 ± 163 | 105 ± 28 | ND | |
*Values from the identical Mak from M. bovis BCG26.
ND - Not determined.