Figure 7. Sensitivity of hTRPM2 variants mutated wihin the NUDT9 domain, to intracellular ADPR and extracellular H₂O₂.

(a), (b) Representative whole-cell patch clamp recordings of hTRPM2 double mutant R1400Q + R1407G. Stimulation was performed (a) with extracellular 10 mM H₂O₂ or (b) with intracellular 0.3 mM ADPR. (c) Corresponding I/V-relation of currents recorded in panel b. (d) Representative whole-cell patch clamp recording of the variant hTRPM2-(+Δ15) that contains the C-terminal part of the NUDT9 domain of nvTRPM2 downstream of the NUDIX box. Stimulation was performed with extracellular 10 mM H₂O₂. Currents were repeatedly inhibited by NMDG.