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. 2014 Dec 9;33(2):539–546. doi: 10.3892/or.2014.3660

Figure 4.

Figure 4

Effect of galanin (GAL) silencing on the proliferation and invasion of colon cancer cells. (A) GAL expression in invasive colorectal cancer (CRC) cells and parental cells by functional separation. GAL expression was significantly higher in invasive CRC cells than parental cells. (B) Cell proliferation ratio in HCT116 and DLD-1 cells. Cell proliferation assay showed a significant decrease in the number of proliferating cells after siRNA-mediated silencing of GAL expression. Proliferation is expressed relative to the parent cells. (C) Hematoxylin and eosin staining of invasive HCT116 and DLD-1 cells transfected as indicated after incubation in the assay chambers for 48 h. (D) The invasiveness of HCT116 cells and DLD-1 cells was significantly decreased after silencing of GAL expression. Parent, non-transfected cells; NC, negative control siRNA.