Figure 2.

Efficacy of ETS-1 transcriptional silencing. Expression level of ETS-1 in the Panc-1 cells was decreased after ETS-1 shRNA transfection. (A) Brightfield and fluorescence images (magnification, ×100). (B) qRT-PCR analysis. Data are presented as mean ± SD of three independent experiments; ^***P<0.001. (C) Lysates from ETS-1 shRNA- and control shNC-transfected cells were subjected to western blot analysis; GAPDH was included as a normalization control. ETS-1, v-ets erythroblastosis virus E26 oncogene homolog 1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.