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TAIL-PCR conditions for the pre-amplification reaction
| Step | Temperature (°C) | Time (min:sec) |
|---|---|---|
| 1 | 93 | 2:00 |
| 2 | 95 | 1:00 |
| 3 | 94 | 0:30 |
| 4 | 60 | 1:00 |
| 5 | 72 | 3:00 |
| 6 | Go to step 3 | 10 times |
| 7 | 94 | 0:30 |
| 8 | 25 | 2:00 |
| 9 | Ramping to 72 | 0.5 °C/s |
| 10 | 72 | 3:00 |
| 11 | 94 | 0:20 |
| 12 | 58 | 1:00 |
| 13 | 72 | 3:00 |
| 14 | Go to step 11 | 25 times |
| 15 | 72 | 5:00 |
| 16 | End |
