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. 2015 Jan 26;5:3. doi: 10.3389/fonc.2015.00003

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Copyright © 2015 Johansson, Tabor, Wikell, Jalkanen and Fuxe.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characteristics of long-term EMT cells. (A,B) Western blot analysis showing more prominent changes in E-cadherin and vimentin expression in EpRas tumor cells after 14 days (B) compared to 48 h (A) of TGF-β1 exposure. Calnexin was used as a internal loading control. (C) Brightfield and immunofluorescence images showing that EpRas cells exposed to TGF-β1 for 14 days acquire characteristic features of EMT including an elongated, fibroblast-like morphology (left panels), loss of E-cadherin at cell–cell junctions (middle panels), and cytoplasmic staining of vimentin (right panels). Scale bars: 20 μm (left panels); 5 μm (middle and right panels).