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. 2015 Jan 23;56(1):598–605. doi: 10.1167/iovs.14-15452

Figure 1.

Figure 1

Comparison between thiol repair enzyme activities in normal nucleus and those in ECCE cataractous lenses. Nuclear portions of nine normal, clear human lenses (Ctl) and whole tissues of human ECCE cataractous lenses of different types of cataracts were used. The cataractous lens samples included 28 cortical (Cor), 26 nuclear (Nuc), 17 mixed cortical and nuclear (Mix), 17 mature cataracts (Mat), and 9 hypermature cataracts (Hyp). Each lens preparation was assayed for GR, TR, TTase, and TRx activities. An aliquot of deproteinized lens homogenate was used for GSH analysis. Data are expressed as means ± SD. *P < 0.05 by comparison with the control. Equal amounts of proteins from the above sample preparation were used for Western blot analysis for the protein content of TTase, GR, and Trx, using specific antibodies for TTase, GR, and Trx. (A) GSH level; (B) GR activity; (C) TTase activity; (D) TR activity; (E) Trx activity; (F) Western blot analysis of TTase, GR, and TRx in human cataractous lenses.