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. Author manuscript; available in PMC: 2015 Jan 26.
Published in final edited form as: Clin Lung Cancer. 2010 Nov 1;11(6):405–411. doi: 10.3816/CLC.2010.n.052

Figure 2. The Demethylation Function of 5-Azacytidine in the NSCLC Cells.

Figure 2

H226 human NSCLC cells (8 × 104 cells) were treated with 5-Aza at 0.1, 1, and 10 ng/mL. The methylation status of the RASSF1a promoter was detected using the EZ DNA Methylation-Direct Kit and methylation-specific PCR. Bands 1 to 3 are samples of water, methylated DNA control, and unmethylated DNA control; bands 4 to 7 are samples of H226 cells treated with 5-Aza at 0, 0.1, 1, and 10 ng/mL, respectively.

Abbreviations: 5-Aza = 5-azacytidine; M = methylated detection; NSCLC = non–small-cell lung cancer; U = unmethylated detection