Table 1. Recoveries of OTA Added to Corn, Wheat, and Rice Samplesa.
VHH phage-based
RT-IPCR (n = 3) |
conventional
ELISA (n = 3) |
|||
---|---|---|---|---|
OTA added (μg/kg) | OTA recovered (μg/kg) | CV (%) | OTA recovered (μg/kg) | CV (%) |
Corn | ||||
0.1 | 0.09 ± 0.01 | 11.1 | 0.08 ± 0.01 | 12.5 |
1 | 0.93 ± 0.09 | 9.7 | 0.89 ± 0.10 | 11.2 |
10 | 12.6 ± 1.07 | 8.5 | 10.94 ± 1.15 | 10.5 |
100 | 100 ± 9.80 | 9.8 | 96.3 ± 10.20 | 10.6 |
Rice | ||||
0.1 | 0.12 ± 0.01 | 8.3 | 0.11 ± 0.01 | 9.1 |
1 | 1.03 ± 0.12 | 11.7 | 0.94 ± 0.09 | 9.6 |
10 | 8.75 ± 0.89 | 10.2 | 8.22 ± 0.98 | 11.9 |
100 | 93.9 ± 11.50 | 12.2 | 91.5 ± 10.70 | 11.7 |
Wheat | ||||
0.1 | 0.08 ± 0.01 | 12.5 | 0.08 ± 0.01 | 12.5 |
1 | 0.94 ± 0.08 | 8.5 | 0.86 ± 0.09 | 10.5 |
10 | 9.12 ± 0.96 | 10.5 | 9.44 ± 1.05 | 11.1 |
100 | 116 ± 10.40 | 9.0 | 103 ± 12.60 | 12.2 |
Determinations were performed by VHH phage-based competitive RT-IPCR and conventional ELISA.