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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Bone. 2014 Feb 1;62:10–21. doi: 10.1016/j.bone.2014.01.019

Figure 7. JAG1-Fc is sufficient to rescue mineralization and osteoblast marker induction.

Figure 7

Jag1CKO MEMM cells were incubated in growth media (GM), osteogenic media in the presence of immobilized IgG (OM+IgG), or osteogenic media in the presence of immobilized Jagged1-Fc (OM+Jag1-Fc) for 16d and stained with alizarin red to analyze mineralization (A-C). The alizarin red stain was solubilized and quantified (E). qPCR revealed the induction of osteoblast differentiation markers in mRNA extracted from the cells. Columns, mean fold change obtained from 3 separate experiments; bars, SEM; *=p<0.05 compared to GM; $=p<0.05 compared to OM+IgG.