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Confocal micrographs and corresponding brightfield images of 4 μM Cy3-labeled biosensor 25 targeting FRα. Uptake occurred in (A) KB, (B) HeLa, and (C) HT-1080 cells after 4 h incubation at 37 °C in folic acid-depleted media. Uptake was blocked in (D) KB, (E) HeLa, and (F) HT-1080 cells preincubated in folic acid containing media.
