Effects of varying concentrations
of Parent on complement activation
in the RPE cell in vitro assay. The ratio of C5b-9/ApoE fluorescence
(±SEM, n = 10) is plotted as a percentage of
the positive control. Parent was tested at concentrations of 1, 10,
and 50 μM (PAR1, PAR10, and PAR50). The concentration of W4A9
was 1 μM. All values are expressed relative to the positive
control. Parent shows no significant difference from the positive
control at 1 μM or 10 μM concentrations. At 50 μM
the effect of Parent is equivalent to that of 1 μM W4A9. Both
Parent at 50 μM and W4A9 at 1 μM are significantly different
than the positive control (p-value of <0.001,
two-tailed Mann–Whitney U test).