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. 2014 Nov 18;26(1):29–38. doi: 10.1021/bc500522k

Scheme 2. RAFT Polymerized PNIPAAm Was Functionalized with TFP to Create an Amine Reactive TFP-PNIPAAm Active Ester.

Scheme 2

Functionalized polymer was subsequently conjugated to Ab to create an Ab-PNIPAAm conjugate. Conjugates were purified through a serial combination of thermal precipitation and ion exchange chromatography.