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Determination of FASN TE kinetic parameters. (A) Expression and purification of recombinant FASN TE: CB, commassie blue staining; IB, Western blot. (B) Lineweaver–Burk plot analysis of recombinant TE using 4-MUH fluorogenic assay. (C) Kinetic analysis. The kinetic parameters of FASN TE were determined by plotting 4-MU product formed (pmol/min) vs the concentration of the 4-MUH substrate. The Km of the protein was determined using a one enzyme model with no weighing of the data.
