Figure 2.

PPIs inhibit FASN TE activity. (A) Fluorogenic assay of 25 top-scoring FDA-approved drugs. The two horizontal lines indicate 100% and 60% TE activity (∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001). (B) Dose-dependent inhibition of TE activity by PPIs. Each plot represents the average of three independent experiments. (C) Average simulated structures of PPIs bound to TE. TE is shown in gold ribbon. Omeprazole, pantoprazole lansoprazole, and rabeprazole are shown as ball and stick in green, blue, pink, and orange, respectively. In each panel, the catalytic triad residues and the residues predicted to interact with each PPI are labeled.