Figure 4.

Mass Spectra of the Intact Yeast eIF3:eIF5 Complex and Its Stable Subcomplexes

(A) Mass spectrum showing the intact pentameric eIF3 (364.4 kDa) and eIF3:eIF5 (409.9 kDa) complexes was recorded.

(B) Stable subcomplexes were identified in the region of the low mass-to-charge ratio, showing single protein subunits (circles) and subcomplexes (triangles) of eIF3 and eIF5. The obtained subcomplexes included g:i, b:i, b:g:i, and c:eIF5.