Figure 2.

Inducible Lineage Tracing of mEGCs

Distribution of confetti⁺ cells in the ileum of tamoxifen-treated Sox10::CreER^T2;R26RConfetti mice at T0 (A), T15 (B), and T90 (C). Boxed areas in (A)–(C) correspond to panels (A′)–(C′), respectively. (D and D′) Confetti⁺ cells in a flat-mount preparation of myenteric ganglia from Sox10::CreER^T2;R26RConfetti mice at T0. Arrows in (D) and (D′) indicate Sox10⁺Confetti⁺ glial cells. Asterisks in (D) indicate the position of confetti-negative enteric neurons. (E) Quantification of VC units with confetti⁺ glial cells at T0, T15, and T90. Data represented as mean ± SEM. Significant differences between the ages have been obtained with the two-way ANOVA, p < 0.0001, Tukey post hoc test, ^∗∗∗∗p < 0.0001 (T0–T15;T0–T90), p = 0.7173 (T15–T90). Although VC units with monochromatic glia showed no significant change in number (p > 0.1), VC units with polychromatic glia showed significant differences: ^∗∗∗∗p < 0.0001 (T0–T15; T0–T90), p = 0.6222 (T15–T90). The F(DFn, DFd) and p values for Factor 1: T0 Vs T15 Vs T90 is 29.94 (2, 18) with p = 0.08; Factor 2: polychromatic Vs monochromatic is 3.36 (1, 18) with ^∗∗∗∗p < 0.0001 and interaction of Factor 1 with Factor 2 is 8.104 (2, 18) with ^∗∗p = 0.0031. cm, circular muscle layer; lm, longitudinal muscle layer; mp, myenteric plexus. Scale bars: 100 μm (A–C); 50 μm (A′–C′); 20 μm (D and D′).