Unaligned length per neurite length ratios (mean±SE) for multiple neuronal types cultured on unpatterned and patterned substrates. A) Neurite alignment from dissociated SGNs, DRGNs, and TGNs is statistically different on patterned substrates compared to unpatterned controls (*p < 0.005, Mann-Whitney Rank Sum test). SGN neurites align more strongly to the pattern than DRGN and TGN neurites (**p < 0.001, one way ANOVA). B) Dendritic neurite (MAP 2-positive) alignment from dissociated CGNs is statistically different on patterned substrates compared to an unpatterned control (*p < 0.005, Mann-Whitney Rank Sum test). However, axonal neurite (Tau-positive) alignment from dissociated CGNs is not statistically different (p = 0.135) from an unpatterned control. Micropatterns used for each neuronal culture have periodicities of 50 μm and channel amplitudes of 3 μm. The number in each bar represents the number of neurites measured. Error bars represent standard error of the mean (SE).