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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Prostate. 2013 Dec 16;74(4):395–407. doi: 10.1002/pros.22761

Figure 3. eHsp90α initiates a pro-inflammatory phenotype in stromal prostate fibroblasts.

Figure 3

A) Serum starved (0.1%) NPFs were treated with Hsp90α protein overnight. CM was collected and analyzed via a cytokine and MMP-based array. B) ELISA confirmation of the Hsp90α-mediated secretion of IL-6, IL-8, and MMP3 in NPFs. C) Similar analysis of Hsp90α-mediated secretion of IL-6, IL-8, and MMP3 in PSC27 following the indicated treatment times. D) Analysis of IL-6 and IL-8 secretion in ARCaPE-LacZ and ARCaPE-eHsp90 in CM, and effects of addition of this CM (1:1) to NPFs (16 hr). Secreted protein values were normalized and are presented as fold change over untreated control. Hsp90α protein significantly induced IL-6, IL-8, and MMP3 secretion (*) as determined by ANOVA and Student’s t-test (p < 0.05). E) RNA was isolated from cells in D and the indicated targets analyzed via qPCR in triplicate.