Skip to main content
. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Prostate. 2013 Dec 16;74(4):395–407. doi: 10.1002/pros.22761

Figure 5. Hsp90α regulates stromal IL-6 and IL-8 secretion via an ERK1/2 and MMP2/9 dependent pathway.

Figure 5

A, B) Serum starved NPFs were pre-treated (6 hr) with either U0126 (10 µM), MMP-2/9 inhibitor IV (1 µM) or NPGA (1 µM) prior to incubation (16 hr) with either Hsp90α protein (A) or F-5 peptide (B). ELISA analysis was used to evaluate secreted levels of IL-6 and IL-8. C) RNA was harvested from the treated cells and transcript levels corresponding to IL-6 and IL-8 were determined by qRT-PCR. Values were normalized to untreated control and significance (*, #) was determined by ANOVA and Student’s t-test (p < 0.05). (#) denotes significance of inhibitory effects of treatments relative to Hsp90 protein or F-5 peptide treated cells.