Figure 6. Effect of Src upregulation and Cav-1 downregulation on growth and lung colonization of UMUC-3 cells.

A) In vivo subcutaneous growth of cells in Figure 5a. 10⁶ cells were injected subcutaneously in 10 mice per group. Mean tumor volume ± SEM (44). *p <0.036 compared to controls at 21 days. B) In vivo lung metastasis by 12p quantitative PCR in mice injected UMUC-3 cells. Inset: Appearance of lungs with metastatic nodules (arrow) found at necropsy. C) In vivo lung metastasis of mice injected 8 weeks previously with Lul-2 cells with or without Y27632 treatment. i) Representative Xenogen images (D-luciferase) of vehicle treated and Y27632 treated animals ii) Representative gross lung pictures at 8 weeks. Arrows indicate lung metastases. iii) 12p quantitative PCR from vehicle treated and Y27632 treated mice at 8 weeks.