TABLE 1.
Real-time PCR primers
| Gene | Forward | Reverse |
| Aacs | CAGCCTGTGTGTACCCCAGTA | CGGCCACCTCCACTTTCTTGC |
| β-actin | GTTCCGATGCCCTGAGGCTC | CAGACAGCACTGTGTTGGCA |
| Acaca | ATCCACAATGCCAACCCTGAG | CCTGGACCAAGCTGCGGATCT |
| Acadm | AGACGAAGCCACGAAGTATGC | TCATCAGCTTCTCCACAGGGT |
| Agpat2 | ACGCAACGACAATGGGGACCT | GGTGGCCCTCATGGACTGGTA |
| Agpat6 | GAGTGAAGTCTGCCATTGCCC | GGCTGCGGTCCTCATGGTTTC |
| Bmal1 | CCTCCCCCTGATGCCTCTTCT | TGCCTCATCGTTACTGGGACT |
| Cd36 | GGAGCTGTTATTGGTGCAGTC | TGCTGTTCTTTGCCACGTCAT |
| Clock | CAGCTTCCTTCAGTTCAGCAG | TACTGTGGCTGGACCTTGGAA |
| Cpt1a | ACTATGTGTCCTGTGGCGGGG | TTTTGGAATTGGCGGTGAGGC |
| Cry1 | AGCAGCAGCGGGAATGGAGGG | CTGGGCATCCTCTTGCTGACT |
| Cry2 | TTGGCATCTGTCCCTTCCTGT | GCCCGCTTGGTCAGTTCTTCA |
| Cyclophilin B | AAGGTGCTCTTCGCCGCCGCC | TGATGACACGATGGAACTTGCTGT |
| Dbp | TGCTAATGACCTTTGAACCTGATCC | TGCATCTCTCGACCTCTTGGCTGCT |
| Dgat2 | CAGCATCCTCTCAGCCCTCC | CAGCCAGGTGAAGTAGAGCA |
| Elovl6 | CTCAGCAAAGCACCCGAACTA | TGACACAGCCCATCAGCATCT |
| Fasn | AGAGCCTGGAAGATCGGGTGG | GCACAGACACCTTCCCGTCAC |
| Gpat1 | CTCTGCTGCCATCTTTGTCCA | AGACACTCGCTTTTGCTTGGT |
| Hmgcr | CAACGCCCACGCAGCAAACAT | CTCACCAGCCATCACAGTGCC |
| Ldlr | GGCAGAGGGAATGAGGAGCAG | GCTCGTCCTCTGTGGTCTTCT |
| Per1 | TGACATACCAGGTGCCGTCCA | CAGTCCACACACGCCATCACA |
| Per2 | CCAGGCGGTGTTGAAGGAGGA | CCTCTATCCTGGGGCCTGTCA |
| Ppara | GGCTGCTATAATTTGCTGTGGAG | TGTGTACGAGCTGCGCATGC |
| Ppard | CCGCAAGCCCTTCAGTGACATCAT | GCAGATGGAATTCTAGAGCCCGCA |
| Pparg | TGCGGAAGCCCTTTGGTGACT | ATGTCCTCGATGGGCTTCACGTTC |
| Rev-erba | CCGCACAACCTACAGTCTGCA | GTTGTTCAGGGTCCGCAGGTC |
| Scd1 | AGCCTGTTCGTTAGCACCTTC | ACTCCCGTCTCCAGTTCTCTT |
| Slc27a2 | TGAGGGTCGAATTGGGATGGC | TTCCATCAGGGTCACTTTGCG |
| Slc27a5 | GCTCCCTGCCTATGCCACACC | CACACACAGCCTGGTACACAT |
| Srebp1c | CGGCGCGGAAGCTGTCGGGGT | TGTCTTGGTTGTTGATGAGCTGGA |
Aacs, acetoacetyl-coenzyme A synthetase; Acaca, acetyl-coenzyme A carboxylase alpha; Acadm, acyl-coenzyme A dehydrogenase, medium chain; Agpat2, 1-acylglycerol-3-phosphate O-acyltransferase 2; Agpat6, 1-acylglycerol-3-phosphate O-acyltransferase 6; Cpt1a, carnitine palmitoyltransferase 1a; Dbp, D site albumin promoter binding protein; Dgat2, diacylglycerol O-acyltransferase 2; Elovl6, ELOVL family member 6, elongation of long chain fatty acids; Gpat1, glycerol-3-phosphate acyltransferase, mitochondrial; Hmgcr, 3-hydroxy-3-methylglutaryl-coenzyme A reductase; Ldlr, low density lipoprotein receptor; Scd1, stearoyl-coenzyme A desaturase 1; Slc27a2, solute carrier family 27 (fatty acid transporter) member 2; Slc27a5, solute carrier family 27 (fatty acid transporter) member 5; Srebp1c, sterol-regulatory element binding protein 1c.