Fig. 8.

DNL is repressed in gonadal WAT of GLS5 mice and negatively correlates with lipid accumulation and adiposity. DNL of gonadal WAT (n = 4–6/group) in 12–15-week-old mice. A: Mean gonadal WAT weights. Lipid synthesis was determined by measuring the incorporation of ³H-H₂O into fatty acids (B) and sterols (C). The experimental procedure and mice are as described in the caption of Fig. 6. D: Relative expression levels of genes encoding for enzymes involved in de novo fatty acid and TG synthesis were measured in gonadal WAT of 4 h-fasted male mice fed a LD for 10 days (n = 5–6/group). E–G: Gonadal WAT fatty acid synthesis was significantly and negatively correlated with liver TG mass (R² = 0.26) (E), gonadal WAT weight (R² = 0.31) (F), and body weight (R² = 0.39) (G), (n = 19). Values are mean ± SEM. *P < 0.05 versus WT, ^#P < 0.05 versus GKO, ^P < 0.05 versus GLS5; one-way ANOVA analysis with Tukey’s post hoc test (A–D) and linear regression analysis (E–G).