Fig. 1.

Expression of G5 and G8 mRNA (A) and protein (B) in the liver and intestine of WT, liver-specific (L-G5G8^−/−), intestine-specific (I-G5G8^−/−), and total G5G8^−/− KO mice. A: RT-PCR was performed to quantitate levels of G5 and G8 mRNA in mice of the designated genotypes (five female mice/group, 12–15 weeks old). Cyclophilin was used as an internal control for mRNA expression. The mean values of the WT mice were set to 1. Standardized means ± SEM are shown. B: Immunoblot analysis of G5 and G8 in membranes isolated from the liver and intestine of mice was performed as described in the Materials and Methods. M, mature; P, precursor. The asterisk indicates a nonspecific band.