Fig. 4.

Levels of selected mRNAs (A) in the jejunum (enterocytes) and proteins in the intestine (B) of WT, G5G8^−/−, L-G5G8^−/−, and I-G5G8^−/− female mice (n = 5/group, 23–25 weeks old). Total RNA was isolated from the jejunum of each mouse and the relative mRNA levels were measured using quantitative real-time PCR as described in the Materials and Methods. Cyclophilin was used as an internal control, and the level was expressed relative to the level of the transcript in the WT animals, which was set to 1. Values are means ± SEMs. B: Enterocytes were isolated from the three sections of intestine as described in the Materials and Methods. Membrane and nuclear fractions were prepared as described in the Materials and Methods. A total of 35 μg of protein was subjected to SDS-PAGE and immunoblotted with Abs to the indicated proteins as described in the Materials and Methods. This experiment was repeated three times, and the results were similar. CNX, calnexin; CREB, cAMP response element binding protein; DHCR24, desmosterol reductase; FPPS, farnesyl pyrophosphate synthase; SCD1, stearolyl-CoA desaturase-1; SS, squalene synthase.