Fig. 5.

Levels of selected mRNAs (A) and proteins (B) in the livers of WT, G5G8^−/−, L-G5G8^−/−, and I-G5G8^−/−female mice (n = 5/group, 23–25 weeks old). Total RNA was isolated from the liver of each mouse, and the relative mRNA levels were measured using quantitative real-time PCR as described in the Materials and Methods. Cyclophilin was used as an internal control, and the level was expressed relative to the level of the transcript in the WT animals, which was set to 1. Values are means ± SEMs. B: Membrane and nuclear fractions were prepared as described in the Materials and Methods. A total of 35 μg of hepatic protein was subjected to SDS-PAGE and immunoblotted with Abs to the indicated proteins as described in the Materials and Methods. This experiment was repeated twice, and the results were similar.