Fig. 6.

Contribution of G5G8 to fecal excretion of cholesterol. A total of 60 µCi of ³H-labeled cholesterol were dried and dissolved in 6 ml of 20% Intralipid (Baxter Healthcare). The lipid mixture was sonicated, and 0.2 ml was injected into the tail veins of six male mice/group (21–24 weeks old). Each mouse received 40 mg of triglyceride and 13 ng of cholesterol (2 μCi). The feces were collected for 3 days, and the fraction of the radiolabeled cholesterol that was injected and appeared as sterol or bile acid in the feces (A) or gallbladder bile (B) was quantitated. This experiment was repeated twice, and the results were similar. Values are means ± SEMs. * P < 0.05, ** P < 0.01, and ^† P < 0.001.