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Kinetics of RA formation by recombinant ALDH1A proteins. Recombinant purified human ALDH1A was used to determine the kinetics of atRA formation by each ALDH1A enzyme (A). Because only ALDH1A1 formed 13-cisRA from 13-cis-retinal, only 13-cisRA formation by ALDH1A1 was characterized (B). The kinetic constants obtained are shown in Table 2.
