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The effect of PGE2 on IP-10 mRNA in LPS-activated adipocytes and SVCs from normal diet or high-fat diet mice. Adipocytes and SVCs separated by collagenase digestion of EP4+/+ adipose tissue were cultured in the presence or absence of PGE2 (50 nM for 1.5 h), and then stimulated with LPS (5 ng/ml for 9 h). RNA then was isolated in each condition and IP-10 mRNA was quantified. N = 6; *, P < 0.05 versus LPS. All data are expressed as means ± SEM.
