Fig. 1.

Impacts of GPR120 gene invalidation on body weight, blood glucose, plasma insulin levels and circumvallate papilla morphology. A: Genotyping of studied mice: agarose gel electrophoresis of PCR products. B: RT-quantitative PCR of CVP (n = 5, ud, undetectable). C: Typical GPR120^−/− phenotypes compared with littermates GPR120^+/+ mice: evolution of body weight in mice fed a standard chow (n = 7), fasted blood glucose and plasma insulin levels (n = 8–12). Means ± SEM. *, P < 0.05; **, P < 0.01. D: Morphology of CVP from wild-type and GPR120^−/− mice. Gross anatomy: hematoxylin-eosin-stained CVP. Black arrows show apical taste pore areas of taste buds. Identification of taste buds cells by α-gustducin staining and visualization of taste bud cells proliferation by Ki67 staining.