Fig. 5.

Effects of LA or GA on Ca²⁺ signaling in mouse circumvallate TBCs. Ca²⁺ imaging studies were performed in calcium-containing (100% Ca²⁺) media. The changes in intracellular Ca²⁺ (F340/F380) were monitored under a Nikon microscope (TiU) by using S-fluor 40× oil immersion objectives, as described in the Materials and Methods section. Colored time-lapse changes show the kinetics of the rise in [Ca²⁺]_i levels in a CD36-positive TBC freshly isolated from CVP from GPR120^+/+ and GPR120^−/− mice following addition of 20 µM LA or GA in the medium. The arrows indicate when LA or GA were added into the cuvette without interruptions in the recording. A: changes (F₃₄₀/F₃₈₀) in intracellular Ca²⁺ evoked by LA, 20 µM and (B) by GA, 20 µM. C: Means ± SEM (n = 7). ***, P < 0.001. ns, nonsignificant.