. 2015 Feb;56(2):390–402. doi: 10.1194/jlr.M055590

TABLE 5.

Kinetic parameters for ARA and DHA in total phospholipids and phospholipid fractions of n-6 PUFA-adequate and n-6 PUFA-deprived rats

Infusate	Lipid Class	Diet	Slope (per Day)	Slope SEM	Loss t_1/2 (Day)	J_out(nmol/g/Day)	Fractional Loss (% per Day)
ARA	Total	Adequate	−0.0096	0.00076	31 ± 2.5	134.7 ± 2.3	2.2
		Deprived	−0.0071^a	0.00044	42 ± 2.6^a	85.4 ± 2.6^a	1.6
	ChoGpl	Adequate	−0.0130	0.00092	23 ± 1.6	43.0 ± 0.6	3.0
		Deprived	−0.0091^a	0.00069	33 ± 2.5^a	24.0 ± 0.3^a	2.1
	EtnGpl	Adequate	−0.0093	0.00071	32 ± 2.5	73.0 ± 0.5	2.1
		Deprived	−0.0068^a	0.00029	44 ± 1.9^a	42.2 ± 1.0^a	1.6
	PtdIns	Adequate	−0.0122	0.00093	25 ± 1.9	10.4 ± 1.6	2.8
		Deprived	−0.0085^a	0.00065	35 ± 2.7^a	6.6 ± 1.1	2.0
	PtdSer	Adequate	−0.0094	0.00104	32 ± 3.6	6.9 ± 0.06	2.2
		Deprived	−0.0069	0.00065	43 ± 4.0	4.0 ± 0.1^a	1.6
DHA	Total	Adequate	−0.0090	0.00079	33 ± 2.9	129.1 ± 11.4	2.1
		Deprived	−0.0086	0.00089	35 ± 3.6	145.7 ± 15.5^a	2.0
	ChoGpl	Adequate	−0.0132	0.00105	23 ± 1.8	31.0 ± 2.5	3.0
		Deprived	−0.0128	0.00109	24 ± 2.0	33.5 ± 2.9^a	2.9
	EtnGpl	Adequate	−0.0108	0.00070	28 ± 1.8	113.5 ± 7.8	2.5
		Deprived	−0.0108	0.00089	28 ± 2.3	121.0 ± 10.2	2.5
	PtdIns	Adequate	−0.0107	0.00112	28 ± 2.9	2.3 ± 0.3	2.5
		Deprived	−0.0102	0.00119	29 ± 3.4	2.4 ± 0.3	2.4
	PtdSer	Adequate	−0.0098	0.00052	31 ± 1.6	39.9 ± 2.1	2.2
		Deprived	−0.0090	0.00098	33 ± 3.6	40.3 ± 4.4	2.1

Loss half-lives were calculated from the slopes of the linear regressions using the equation t_1/2 = log₁₀2/(slope of the regression line). Baseline fatty acid concentrations were used to calculate the rates of loss (J_out) from the different fatty acid pools using the equation J_out = 0.693C_FA/t_1/2, where C_FA is the baseline brain total phospholipids and fractions fatty acid concentration after 15 weeks of feeding. Data are mean ± SEM. Differences in slope and J_outwere assessed with an ANOVA and Student’s t-test, respectively.

P < 0.05 versus adequate n-6 PUFA.