TABLE 2.

Assay validation parameters for 27OHC3S

	Nanograms per Milliliter (Spiked Concentration)	Mean (Concentration Measured)	SD	Precision (% CV)	Accuracy (% RE)
Intra-assay (n = 5)	5	4.9	0.6	11.3	−1.4
	50	52.8	2.2	4.2	5.6
Inter-assay (n = 12)	5	4.9	0.7	14.8	−2.4
	50	52.3	3.4	6.4	4.5
Percent Recovery	5	94.2
	50	100.6
Linearity, LOQs, LOD (ng/ml)		Linearity	LOD	LLOQ	ULOQ
		2.5–500	1	2.5	500
Matrix effect study (regression line)		y = 0.975x + 0.018 (R2 = 0.999) (2.5–500 ng/ml)

CV, Coefficient of variation; RE,: relative error; LLOQ, lower limit of quantification; ULOQ, upper limit of quantification.