Fig. 5.

Normal-phase HPLC profiles of extracts from incubation mixtures of zeaxanthin and β-cryptoxanthin with postmitochondrial fraction of mouse liver. The reaction mixtures containing zeaxanthin (A) and β-cryptoxanthin (B) were incubated at 37°C for 60 min in the standard condition. Peaks were identified as 3-hydroxy-β,ε-caroten-3′-one (peak 1), all-trans-zeaxanthin (peak 2), β,β-caroten-3-one (peak 3), β,ε-caroten-3′-one (peak 4), and all-trans-β-cryptoxanthin (Peak 5). UV-VIS spectra of the peaks in the HPLC mobile phases are shown in (C). The three-digit numbers attached to the spectral curves indicate the wavelength (in nanometers) of absorption peaks.