TABLE 1.
Oxidation of xanthophylls by postmitochondrial fraction of mouse liver
Substrate | Complete Incubation | Minus NAD+ Control | |
Product (μM) | Residual Substrate(μM) | Residual Substrate (μM) | |
β-Cryptoxanthin | β,ε-Caroten-3′-one | ||
0.274 ± 0.007 | 3.75 ± 0.08 | 13.74 ± 0.12 | |
Lutein | 3′-Hydroxy-ε,ε-caroten-3-one | ||
0.700 ± 0.017 | 7.17 ± 0.55 | 14.03 ± 0.30 | |
Zeaxanthin | 3-Hydroxy-β,ε-caroten-3′-one | ||
0.170 ± 0.006 | 3.13 ± 0.25 | 13.78 ± 0.13 | |
3-Hydroxy-β,ε-caroten-3′-one | ε,ε-Carotene-3,3′-dione | ||
0.408 ± 0.019 | 9.66 ± 0.31 | 15.16 ± 0.23 | |
Lactucaxanthin | 3′-Hydroxy-ε,ε-caroten-3-one | ||
0.014 ± 0.007 | 16.53 ± 1.43 | 13.13 ± 0.33 | |
3′-Hydroxy-ε,ε-caroten-3-one | ε,ε-Carotene-3,3′-dione | ||
0.011 ± 0.003 | 17.30 ± 0.31 | 16.38 ± 0.13 |
Xanthophyll (20 μM) was incubated at 37°C for 60 min in the standard reaction mixture. Values are mean ± SD of triplicate incubations.