Table 1.
Intrapipette | Bath | ||||||||||||
1 | 2 | 3 | 4 | 5 | 6 | 7 | |||||||
CsF | 135 | KF | 135 | NaF | 130 | NMDG-F | 130 | NMDG | 115 | NMDG | 115 | NMDG | 135 |
NaCl | 5 | NaCl | 5 | NaCl | 5 | NaCl | 5 | NaCl | 20 | NaCl | 20 | NaCl | 2 |
EGTA | 10 | EGTA | 10 | EGTA | 10 | EGTA | 10 | CsCl | 2 | KCl | 2 | Ca(OH)2 | 2 |
HEPES | 10 | HEPES | 10 | HEPES | 20 | HEPES | 10 | Ca(OH)2 | 2 | Ca(OH)2 | 2 | TEA-Cl | 10 |
HEPES | 10 | HEPES | 10 | HEPES | 20 | ||||||||
TEA-Cl | 10 | TEA-Cl | 10 | Nif | 1 | ||||||||
Nif | 1 | Nif | 1 |
(Solutions 1–7) Compositions of the intrapipette and bath solutions (mM) used to record whole-cell Na+ currents (1, 5) and gating pore currents (1–7). Solutions 1 and 5 were used to record gating pore current selective for Cs+. Solutions 2 and 6 were used to record gating pore currents selective for K+. Solutions 3 and 7 were used to record gating pore currents selective for Na+. Solutions 4 and 5 were used to evaluate the permeation of NMDG. Ca(OH)2, calcium hydroxide; CaCl2, calcium chloride; CsF, cesium fluoride; EGTA, ethylene glycol tetra acetic; KCl, potassium chloride; MgCl2, magnesium chloride; NaCl, sodium chloride; Nif, niflumic acid; NMDG(-F), N-methyl-d-glucamine (fluoride); TEA-Cl, tetraethyl ammonium chloride.