Table 1.

Compositions of solutions

Intrapipette								Bath
1		2		3		4		5		6		7
CsF	135	KF	135	NaF	130	NMDG-F	130	NMDG	115	NMDG	115	NMDG	135
NaCl	5	NaCl	5	NaCl	5	NaCl	5	NaCl	20	NaCl	20	NaCl	2
EGTA	10	EGTA	10	EGTA	10	EGTA	10	CsCl	2	KCl	2	Ca(OH)2	2
HEPES	10	HEPES	10	HEPES	20	HEPES	10	Ca(OH)2	2	Ca(OH)2	2	TEA-Cl	10
								HEPES	10	HEPES	10	HEPES	20
								TEA-Cl	10	TEA-Cl	10	Nif	1
								Nif	1	Nif	1

(Solutions 1–7) Compositions of the intrapipette and bath solutions (mM) used to record whole-cell Na⁺ currents (1, 5) and gating pore currents (1–7). Solutions 1 and 5 were used to record gating pore current selective for Cs⁺. Solutions 2 and 6 were used to record gating pore currents selective for K⁺. Solutions 3 and 7 were used to record gating pore currents selective for Na⁺. Solutions 4 and 5 were used to evaluate the permeation of NMDG. Ca(OH)₂, calcium hydroxide; CaCl₂, calcium chloride; CsF, cesium fluoride; EGTA, ethylene glycol tetra acetic; KCl, potassium chloride; MgCl₂, magnesium chloride; NaCl, sodium chloride; Nif, niflumic acid; NMDG(-F), N-methyl-d-glucamine (fluoride); TEA-Cl, tetraethyl ammonium chloride.