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. 2014 Dec 9;143(2):398–407. doi: 10.1093/toxsci/kfu240

FIG. 2.

FIG. 2.

Dose-response relationships for effects of pregnenolone on the mRNA expression of pxr (A), ahr2 (B), CYP1A (C), CYP2AA1 (D), CYP2AA2 (E), CYP2AA12 (F), CYP3A65 (G), and CYP3C1 (H) in developing zebrafish (determined at 72 hpf). Embryos were exposed to carrier (0.1% DMSO) or differing concentrations of pregnenolone (PN; 1, 3 or 10 µM) for 24 h starting at 48 hpf. At 72 hpf, eleutheroembryos were sampled for quantitative real-time PCR analysis. Relative expression (fold-control) was calculated by E−ΔΔCt using arnt2 as a reference gene. Statistical differences between control and treatment group were determined by one-way ANOVA followed by Dunnett’s multiple comparison test and are shown by asterisks (*p < .05 and **p < .01, ***p < .001, n = 4).