Table 3. The details of ion score obtained by Mascot search using peak lists from two different peak detection tools.
| Spectral information | Mascot search score | |||
|---|---|---|---|---|
| Well # | Precursor ion (Da) | Peptide | MWD list | Distiller list |
| 17 | 1072.648 | SHCIAEVEK | 24 | Xb) |
| 23 | 1443.798 | YICDNQDTISSK | 68 | X |
| 30 | 1674.062 | QEPERNECFLSHK | 19 | X |
| 35 | 1927.895 | CCAADDKEACFAVEGPK | 36 | X |
| 40 | 1554.788 | DDPHACYSTVFDK | 23 | 16 |
| 43 | 1305.760 | HLVDEPQNLIK | 31 | X |
| 43 | 1576.932 | LKPDPNTLCDEFK | 18 | X |
| 44 | 927.463 | YLYEIAR | 50 | 48 |
| 45 | 1640.114 | KVPQVSTPTLVEVSR | 69 | 69 |
| 46 | 1512.013 | VPQVSTPTLVEVSR | 54 | X |
| 49 | 1881.019 | RPCFSALTPDETYVPK | 41 | 19 |
| 50 | 927.436 | YLYEIAR | [32]a) | [31] |
| 51 | 1163.495 | LVNELTEFAK | 59 | 46 |
| 53 | 1439.835 | RHPEYAVSVLLR | 57 | 30 |
| 56 | 1283.899 | HPEYAVSVLLR | 47 | 23 |
| 56 | 1419.878 | SLHTLFGDELCK | 66 | 71 |
| 59 | 927.557 | YLYEIAR | [23] | X |
| 59 | 1479.976 | LGEYGFQNALIVR | 31 | X |
| 63 | 927.698 | YLYEIAR | [27] | [20] |
| 63 | 1419.950 | SLHTLFGDELCK | [44] | [12] |
| 70 | 2045.571 | RHPYFYAPELLYYANK | 11 | X |
| 70 | 1480.156 | LGEYGFQNALIVR | X | 17 |
| 72 | 1567.923 | DAFLGSFLYEYSR | 42 | X |
| Total score (Coverage) | 746 (33%) | 338 (16%) | ||
a) The score in bracket represents duplicate peptide from the search results. b) Symbol (X) indicates that the match of peptide was not found from the peak list.