Table 2.
Serology and neutralization of Gulu survivors. Summary of ELISA immunoreactivity, and PRNT50, in human survivor sera from Gulu (S1–S6), and non-infected controls (N1–N4), against the different viral recombinant proteins of SUDV and the whole viral antigen.
| Serology | PRNT50 | ||||||
|---|---|---|---|---|---|---|---|
| VP30 | VP35 | VP40 | NP | GP1–649 1 | SUDV Whole Ag | SUDV | |
| S-1 | ++ | - | + | +++ | +++ | +++ | +++ |
| S-2 | + | - | + | +++ | +++ | +++ | + |
| S-3 | + | + | - | +++ | +++ | ++ | +++ |
| S-4 | +++ | - | - | + | +++ | +++ | +++ |
| S-5 | ++ | - | + | +++ | +++ | ++ | - |
| S-6 | +++ | - | +++ | +++ | +++ | +++ | ++ |
| N-1 | - | - | - | - | - | - | - |
| N-2 | - | - | - | - | - | - | - |
| N-3 | - | - | - | - | - | - | - |
| N-4 | - | - | - | - | - | - | - |
¹ A purified recombinant protein containing the 649 amino terminal amino acid of SUDV GP without the trans-membrane domain. S—Ebola survivors, N—Non-infected control ELISA and neutralization assays results were divided into low (+), medium (++) and strong (+++) immunoreactivity or neutralization capability. For ELISA: (+)—(Lower than 2x cut off value), (++)—(2x cut off value—4x cut off value), (+++)—(Greater than 4x cut off value). For neutralization assay (PRNT50): (+)—(Neutralizes at 1:20 dilution), (++)—(Neutralizes at 1:40 dilution), (+++)—(Neutralizes at greater than 1:80 dilution).