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. 2015 Jan 16;7(1):180–198. doi: 10.3390/v7010180

Figure 3.

Figure 3

Pentosan Polysulphate Inhibition of PrPSc in L929 and Elk21- Cells. (A) L929 cells were exposed to serial dilutions of RML, 22 L and ME7 in the presence of 10 μg/mL pentosan polysulphate (PPS). PPS significantly attenuated PrPSc generation in both 0.1% and 0.01% RML, 22L and ME7 brain homogenate (BH) treatments compared to control (### p < 0.001). PPS displayed a significantly greater inhibitory effect in the 0.01% BH treatments compared to the 0.1% BH treatments (*** p < 0.001). Control: RML 0.1% BH; (B) Elk21- cells were exposed to serial dilutions of 2 different CWD isolates (elk; 132 M, and white-tailed deer; 95Q/96G) in the presence of 10 μg/mL PPS. Control: Elk or white-tailed deer CWD 0.1% BH. The generation of PrPSc was determined by ELISPOT analysis following three passages of the cells. PPS significantly attenuated PrPSc spot generation in Elk21- cells when compared to control (*** p < 0.001 vs. control). There was no significant difference between 0.1% and 0.01% CWD BH in the presence of PPS. N = 6, mean ± SEM.