Skip to main content
. 2014 Dec 23;12(1):64–82. doi: 10.3390/ijerph120100064

Figure 5.

Figure 5

Inhibition of LOX promoter activities in NNK-treated cells. (A) Schematic representation of LOX promoter-reporter chimera with the Inr-DPE core promoter. (B) NNK inhibition of LOX promoter activities in transfected cells. RFL6 cells were transiently co-transfected with the Prom-804 construct and the pRL-TK vector, an internal control, then treated with NNK at indicated concentrations for 48 h. Luciferase activities in cell lysates were measured by luminometry. Firefly luciferase activities elicited by the LOX promoter were normalized to Renilla luciferase activities derived from the pRL-TK vector and expressed as relative luciferase activities as instructed by manufacturer (Promega). Data shown are the mean ± SD (n = 3). * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001 compared with the control (100%).