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. 2014 Dec 23;12(1):64–82. doi: 10.3390/ijerph120100064

Figure 7.

Figure 7

Enhancement of methylation at the LOX promoter region in NNK-treated cells as determined by using the methylation promoter PCR kit. The same amount of genomic DNA isolated from control and NNK-treated cells were digested with restriction enzyme Mse I, then incubated with MBP to form a protein/DNA complex. Methylated DNA was isolated using a separation column and amplified by PCR. PCR products as a 205 bp DNA were analyzed on 2.2% agarose gels and densities of DNA bands measured by the 1D Scan software. One typical gel among three repeated experiments is presented.