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. 2015 Jan 27;5:8055. doi: 10.1038/srep08055

Figure 3. Colony-forming assay.

Figure 3

Bone marrow mononuclear cells were isolated from mice soon (A,C) or 3 months (B,D) after daily radiation exposures to 2, 10, 50, and 250 mGy γ-ray, and then cultured in methylcellulose complete medium. The colony formation was observed under microscopy at 9 days after incubation. The number of all types of colonies (>30 cells) and the mixed colonies (at least two types of cells in colony) were counted, and data was represent the mean of duplicate assays. * p<0.05 vs control; ** p<0.01 vs control.