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Disc diffusion assays on QSI-reporter strain C. violaceum ATCC 12472 using the crude organic extract of marine isolate KS8. A disc loaded with 60 μL of EtOAc and allowed to air-dry was included in the plate as a solvent control (top). Two discs were loaded with 40 μL of the crude organic extract of marine isolate KS8 (10 mg/mL). QSI activity was detected as an opaque halo surrounding the test discs.
