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. 2014 Dec 25;13(1):48–64. doi: 10.3390/md13010048

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© 2014 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Double staining for fucoidan and ED1 in the livers of BBN + 2% fucoidan rats. (A) A representative case of single staining for fucoidan. Fucoidan stained dark brown in Kupffer cells in the liver of a BBN + 2% fucoidan rat (white arrows); (B) A representative case of single staining for ED1. ED1 stained pink in Kupffer cells in the liver of a BBN rat (head arrows); (C) A representative case of double staining for fucoidan and ED1. Some Kupffer cells in the sinusoids of hepatic lobules from BBN + 2% fucoidan rats were positive for both fucoidan and ED1 staining (red arrows). Bars: 50 μm.