Figure 2.

Effect of 1k and 4c on the cell cycle distribution and apoptosis of HCT-116 cells. Flow cytometric analysis of propidium iodide-stained cells (A) and percentage of Annexin V/propidium iodide (PI) double-stained cells (B), as determined by flow cytometry after 24 h treatment with the compounds or vehicle alone (control). The percentage of cells in the different phases of the cycle or of apoptosis was calculated by Expo32 software. Values are the mean ± SD of three separate experiments in triplicate.