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. 2015 Jan 16;13(1):460–492. doi: 10.3390/md13010460

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Nortopsentin analogue 4c induces accumulation of acid vacuoles in HCT-116 cells. (A) Micrographs of Giemsa-stained cells in 40× magnification. Control, cells treated with vehicle. Representative images of three experiments with comparable results; (B) The percentage of bright red (FL3) fluorescence-positive cells after Acridine Orange (AO) staining and fluorescence-activated cell sorting (FACS) analysis. Results are the mean ± SD of two triplicate experiments. Mean values with unlike superscript letters were significantly different (p < 0.05; Bonferroni’s test).